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Fig. 9. The ovarian epithelium culture technique. To remove oocytes of previtellogenic and vitellogenic stage, ovarian fragments of carp were excised and treated with enzymes. The residual ovarian fragments were precultured for one month. After preculture, ovarian explants were cultured in media with or without 1 ng/ml DHP for 14 days. Small arrows indicate oogonia. Photographs of 1 month culture is originally published in Miura et al., A Progestin and Estrogen Regulate Early Stages of Oogenesis in Fish, Biology of Reproduction 77, 822–828, Fig. 7, 2007.

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