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Fig. 6. Expression of GFP gene in germ-somatic cell pellets after electroporation. Transient expression of transfected genes was examined two days after the electroporation of GFP cDNA into germ-somatic cell pellets. A train of eight square pulses (60 V; duration 50 msec; interval 950 msec), resulted in widespread expression of GFP fluorescence in many round germ cells and somatic cells. CMV, cytomegarovirus promoter; GFP, green fluorescent protein. Reprinted with permission of John Wiley & Sons, Inc. from Molecular Reproduction and Development, 74, Miura et al., Transfer of spermatogenesis-related cDNAs into eel testis germ-somatic cell coculture pellets by electroporation: methods for analysis of gene function, 420–427, Fig. 1, © 2007, Wiley-Liss, Inc., a Wiley Company.

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