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Fig. 4. The schema of the method of germ-somatic cells coculture system. Immature eel testes were enzymatically dissociated and the cell suspension was filtered through meshes and centrifuged in Nycodenz gradients. Separated cell suspension was centrifuged to make pellets and they were cultured with or without 11-KT. After 30 days culture, many spermatozoa (white arrows) having one or two flagella were observed around the pellet of germ cells and somatic cells. Reprinted with permission from Kaiyo to Seibutsu, 24, Miura and Miura, The challenge of artificially producing sperm and egg from immature gametes in vitro, 114–119, Fig. 4, © 2002, Seibutsukenkyusha.

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