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Fig. 3. The eel testicular organ culture system. Freshly removed immature eel testes were cut into small pieces, which were placed on floats of elder pith covered with a nitrocellulose membrane in 24-well plastic tissue-culture dishes (upper left). By using this system, 11-KT can induce the entire process of spermatogenesis for 36 days. Each symbol indicates: GA, type A spermatogonia; GB, type B spermatogonia; SC, spermatocytes; ST, spermatid; SZ, spermatozoa. Bar, 10 μm. Reprinted with permission from Handbook of Animal Cell Technology (Edited by Japanese Association for Animal Cell Technology), Miura and Miura, 287–289, Fig. 15.6, © 2000, Asakura Publishing Co., Ltd., reprinted with permission from Kaiyo to Seibutsu, 24, Miura and Miura, The challenge of artificially producing sperm and egg from immature gametes in vitro, 114–119, Fig. 2, © 2002, Seibutsukenkyusha, and reprinted with permission from Zoological Science, 18, Miura and Miura, Japanese eel: a model for analysis of spermatogenesis, 1055–1063, Fig. 2, © 2001, Zoological Society of Japan.

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