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Table 5. Effects of the hemocyte layers on the adhesion of the mantle epithelial cells. With kind permission from Springer Science + Business Media: In Vitro Cell. Dev. Biol., Monolayer formation and DNA synthesis of the outer epithelial cells from pearl oyster mantle in coculture with amebocytes, 34, 1998, 486–491, Awaji and Suzuki, Table 1.

aThe mantle epithelial cells and hemocytes were inoculated at 1.0 × 106 and 6.1 × 106 cells/well, respectively. In A, the epithelial cells were inoculated to the wells without the hemocytes. In B and C, the hemocyte layers were prepared in the wells. Hemocytes were fixed with formaldehyde in the case of C. The epithelial cells were inoculated onto the hemocyte layers. The wells without the epithelial cells served as the controls. b×106 cells/well. cMean ± standard error; n = 3. dThe enumeration with crystal violet/citric acid solution required liberation of the stained cell nuclei, but only a small portion of the fixed hemocytes liberated the cell nuclei.

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