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Fig. 30. Detection of DNA synthesis in epithelial cell sheet. The cultures on day 6 were labeled with BrdU for 24 h and immunohistochemically stained with anti-BrdU antibody to visualize DNA synthesis in the cells. A part of the immunoreactive nuclei is indicated by black arrowheads. White arrowheads indicate nuclei without positive reactions. A–C - the epithelial cell sheet with different sizes and labeling frequency. D - the epithelial cell sheet without positive reactions. Scale bars - 100 μm. A and B are reprinted with kind permission from Springer Science + Business Media: In Vitro Cell. Dev. Biol., Monolayer formation and DNA synthesis of the outer epithelial cells from pearl oyster mantle in coculture with amebocytes, 34, 1998, 486–491, Awaji and Suzuki, Fig. 4.

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