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Fig. 23. Histological structures of the separated outer epithelia. A - binocular observations showing the folds of the epithelium. B - cross section of the epithelium showing a monolayer structure. a - apical surface with microvilli; b - basal surface with some connective tissue cells. C - transmission electron microscopy of the epithelium. Intercellular attachment between the epithelial cells (arrowheads) remained intact after the dispase digestion. m - mitochondria; n - nucleus; rER - rough endoplasmic reticulum. Scale bars - 500 μm (A), 50 μm (B) and 3 μm, respectively. B is reprinted with permission from Invertebrate Cell Culture, Awaji, Primary culture techniques for the outer epithelial cells of pearl oyster mantle, 239–244, Fig. 2C, © 1997, Science Publishers.

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