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Fig. 19. Polygonal frame-like structures observed under the outgrowth zone. Mantle fragments were cultured for 2 months with the TC199-based medium. A - frame-like structures (arrowheads) on the bottom of a culture vessel with covering cell sheets. B - frame-like structures after removal of the covering cells. Phase contrast. Scale bars - 200 μm (A) and 100 μm (B), respectively. B is reprinted with permission from Biomineralization (BIOM2001): Formation, Diversity, Evolution and Application. Machii, Pearl formation studies in vitro, 133–136, Fig. 4 left, © 2003, Tokai University Press.

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