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Fig. 14. Fragment culture of pearl oyster mantle with the hemolymph-based medium. A–C - epithelial-like cells maintained in culture conditions for 37 days (A), 49 days (B) and 80 days (C) attaching to the bottom of culture vessels. D - membranous structures with some minute precipitates (arrowheads) observed under an outgrowth zone of the culture on day 112. The minute precipitates suggest initiation of crystal growth. m - mantle fragment. Scale bars - 400 μm (B, D) and 200 μm (A, C), respectively.

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