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Fig. 13. Fragment culture of pearl oyster mantle with the hemolymph-based medium. A - active outgrowth of mantle cells from a fragment on day 49. B, C - active outgrowth of round epithelial-like cells on day 7 (B) and day 8 (C). D, E - outgrown epithelial-like cells attaching to the bottom of culture vessels on day 11 (D) and day 19 (E). F - round epithelial-like cells on day 26 possessing nuclei with a prominent nucleolus (arrowhead). m - mantle fragment. Scale bars - 1 mm (A), 400 μm (B, C, E), 200 μm (D) and 100 μm (F), respectively. D is reprinted with permission from Shinju Monogatari, Popular Science, 124, Machii, 161 pp., Fig. 6.14 right, © 1995, Shokabo Publishing. E is reprinted with permission from Protein, Nucleic acid and Enzyme, 34, Machii, Tissue culture of shell, 193–196, Fig. 3, © 1989, Kyoritsu Shuppan.

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