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Fig. 3. Histological changes in mantle allografts and pearl-sac epithelia. A - hemocyte layers (arrowheads) encapsulating the implanted bead and surrounding testis (t) at 2 days after implantation (day 2). The bead was originally at b but removed for tissue sectioning. B - a mantle allograft (a) and surrounding testis (t) on day 2. Arrowheads indicate epithelial cells flattening at the periphery of the graft. C - a mantle allograft (a) on day 4. An arrowhead indicates flattening epithelial cells. D - a pearl-sac epithelium (arrowheads) emigrating from the allograft on day 4. E - a pearl-sac epithelium (arrowheads) on day 14. An arrow indicates acidophilic granules beneath the epithelium. F - mucous cells (m) and cells with acidophilic granules (arrows) in a pearl-sac epithelium (arrowheads) on day 21. b - the place in which the bead was located; o - ovary; t - testis. Mayer's hematoxylin and eosin stain. Scale bars - 160 μm (B, C), 80 μm (A, D), 40 μm (E, F), respectively. B is reprinted with permission from Fish. Sci., 61, Awaji and Suzuki, The pattern of cell proliferation during pearl sac formation in the pearl oyster, 747–751, Fig. 1a, © 1995, The Japanese Society of Fisheries Science.

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