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Fig. 23. Induction of CMA in the muscles of bluefin tuna. The burnt tuna meat showed induction of CMA and extensive proteolysis (Yamashita 2010). Three individuals with normal, burnt, and severely (heavy) burnt muscles were used for the assay. Upper panel: SDS-PAGE on the water-soluble fraction. Lower panel: western blot of the lysosomal fraction obtained by subcellular fractionation with antibodies against cathepsin L, aldolase, and HSC70. The red arrow indicates proteolytic products found in the burnt tuna muscle. A: Internal portion in the white muscle. B: Lateral portion in the white muscle. C: Dorsal portion of the white muscle. D: Dark muscle.

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