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Fig. 20. Induction of Smad2 phosphorylation in the transgenic zebrafish embryos at 9 hpf by whole-mount staining with antibodies vs. phosphorylated Smad2 and HSP70. The embryos overexpressing platyfish HSP70 under the control of the HSC70 promoter (Yamashita and Hojo 2004) were fixed with 10% formalin in PBS, and phosphorylated Smad2 and HSP70/HSC70 were stained with anti-phosphorylated Smad2 rabbit monoclonal antibody (Cell Signaling) and anti-bovine HSC70 (Sigma-Aldrich) by detection of signals with BM Blue POD substrate (Roche Japan).

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