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Fig. 14. Expression of the β-galactosidase gene under the control of the hsp70 promoter. Each 72-h-stage transgenic zebrafish of the F1 generation was fixed and stained with X-gal (Yamashita 1999). The control transgenic fish (control) was cultured at 28.5°C for 72 h after fertilization. The DNA construct of the hsp70 promoter-lacZ gene was microinjected into the cytoplasm of the one-cell-stage embryo. The injected embryos were cultured for 5 days in sterilized tap water in an incubator at 28.5°C, and juvenile fish were then cultivated to adult fish. The transgenic F1 generation was treated with 100 μM sodium arsenite for the last 8 h (As) or heat-shocked in warm water at 37°C for the last 6 h (HS). The tissue-specific expression of the transgene was induced in the lens and yolk extension by sodium arsenite.

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