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Fig. 12. Transactivation activities of zebrafish HSF. (A) Gene expression induced by overexpression of HSF. The cRNA encoding zebrafish HSF (HSF, lane 2) and the HSF mutants, HSF-Del1 (Del1, lane 3) and HSF-Del2 (Del2, lane 4), was introduced into zebrafish embryos at the one-cell stage. The embryos were then incubated at 28.5°C for 12 h. The control embryos were maintained at 28.5°C (control, lane 1), and the heat-shocked embryos were incubated at 37°C from the 10 h post-fertilization (hpf) to the 12 hpf (heat shock, lane 5). In the 12-h embryos, the mRNA expression levels of hsp70-a, hsp47, hsc70-1, and hsf were examined by RT-PCR. (B) The cRNA encoding zebrafish HSF or that of the truncated mutants, HSF-Del1 (Del1) and HSF-Del2 (Del2), was microinjected into zebrafish embryos at the one-cell stage, along with the hsp70-GFP gene construct. The induction of GFP expression under the control of the hsp70 promoter was observed in 12-h embryos by fluorescence microscopy.

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