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Fig. 11. Expression of hsf mRNA by in situ hybridization. The hsf gene was expressed after the gastrula period. The hsf gene is induced by zygotic expression during early development, similar to most other housekeeping genes. hsf mRNA was not observed in embryos within 3 h of fertilization (A) as shown by Northern blot analysis (see Fig. 8). After the gastrula period, hsf mRNA was observed throughout the developmental stages of zebrafish embryos (B)–(D). In 2-day-old embryos, HSF mRNA was highly expressed in the brain, notochord (nt), otic vesicle (ov), and yolk sac (yo) (D). Thus, the spatiotemporal expression of zebrafish hsf mRNA accounts for the tissue-specific heat-inducibility of HSP70 under stress conditions.

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