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Fig. 10. Heat-inducible expression of the hsp70 gene detected by in situ hybridization. Most notably, strong expression of the hsp70 gene was observed in the brain, notochord, and yolk sac of heat-shocked 1- to 2-day-old embryos. In the case of the 2-day-old heat-shocked embryos, hsp70 mRNA expression was elevated in the forebrain, hindbrain, notochord (nt), otic vesicle (ov), yolk sac (yc), and skeletal muscle (m) in the middle part of the body. Thus, the hsp70 gene was expressed in stage- and tissue-specific manners.

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