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Fig. 9. Heat-inducible expression of the hsp70 gene detected by in situ hybridization. The fish hsp70 gene showed stress-inducible gene expression. In zebrafish embryos, hsp70 mRNA was expressed at the segmentation stage after the gastrula period under heat-shock conditions. During the cleavage and blastula periods, no apparent expression of hsp70 mRNA was seen, even in the heat-shocked 3-h embryos (B). However, heat-shock induction of hsp70 mRNA was found in the embryos 6 h after fertilization (D). A temperature shift from 28.5°C to 37°C for 1 h induced hsp70 mRNA expression throughout the entire embryo during the gastrula and segmentation periods (B), (D). Conversely, control embryos cultured at 28.5°C showed no apparent expression of hsp70 mRNA in any of the stages tested in this study (A), (C).

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