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Fig. 8. Northern blot analysis of hsp70 and hsf mRNAs from zebrafish embryos. Total RNA was isolated from embryos incubated at 28.5°C and heat-shocked embryos that had undergone a 1-h temperature shift to 37°C, and then subjected to Northern analysis. (A) hsp70 mRNA, (B) hsf mRNA. The hsf gene was expressed as a primary transcript of 6 kb and a mature form of 2 kb after the gastrula stage, which coincided with the expression pattern of the hsf gene. Embryonic staging was according to Kimmel et al. (1995).

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