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Fig. 1. Expression of HSP70 in the heat-shocked zebrafish embryo. Embryos maintained at 28.5°C were transferred to 37°C for 1 h. The embryos were fixed with 10% formalin in PBS, and HSP70 was stained with a specific monoclonal antibody (Embiotech Laboratories, Tokyo, Japan) vs. heat-inducible HSP70 raised against the C-terminal region of zebrafish HSP70-a by detection of signals with BM Blue POD substrate (Roche Japan, Tokyo, Japan). (A) Whole mount immunostaining showed HSP70 expressed in tissue specific manners. (B) Western blotting.

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