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Fig. 18. Vitellogenin (VG) mRNA levels in Marsupenaeus japonicus ovarian fragments. (A) Vitellogenin levels in 20-h-incubated fragments and unincubated fragments from the same animals. Vitellogenin mRNA levels in the incubated group were significantly higher than those in the unincubated group (mean ± SEM; n = 9, *P < 0.05). (B) Effects of sinus gland extracts on vitellogenin mRNA levels. Vitellogenin mRNA levels are expressed as percentage changes relative to control values (mean ± SEM; n = 4). Unincubated samples were prepared from ovarian fragments used for incubation at the highest dose. *P < 0.05; **P < 0.01. Reprinted from General and Comparative Endocrinology, 144, Tsutsui et al., The effects of crustacean hyperglycemic hormone family peptides on vitellogenin gene expression in the kuruma prawn, Marsupenaeus japonicus, 232–239, Copyright (2005), with permission from Elsevier.

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