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Fig. 14. VgR cloning strategy and sequence for Marsupenaeus japonicus. (A) Cloning strategy of Mar. japonicus ovarian LDLR cDNA. Solid black triangles indicate the primers designed based on the sequence of Penaeus semisulcatus putative ovarian lipoprotein receptor cDNA. Open triangles indicate the primers designed based on the sequence of Mar. japonicus ovarian LDLR. Arrows indicate the universal primers for 5' and 3'-RACE. Meshwork shows the ligand binding domain (LDL-receptor class A); gray color shows the EGF precursor domain (LDL-receptor class B); The horizontal striped section shows an EGF-like domain; a capital T shows a transmembrane region. (B) Nucleotide sequence of ovarian LDLR cDNA from Mar. japonicus and its deduced amino acid sequence. This nucleotide sequence has been deposited in the DDBJ database (accession number AB304798). Shadowed areas indicate the ligand binding domain (LDL-receptor class A), thinly-outlined rectangles indicate the EGF precursor domain (LDL-receptor class B), the double underline indicates an EGF-like domain and the dotted underline indicates a transmembrane region. Three bold serial circles (SDE) indicate highly conserved residues in the LDL receptor family. Potentially related sequences of SDE are shown in thinly-outlined serial circles. Bold letters indicate YWTD repeats and its potentially related sequence. The single underlines indicate residues homologous to the internalization signal (NPXY). A pair of solid black arrowheads indicates the 5' upstream probe region, and open arrowheads indicate the 3' downstream probe region used for library screening. Thickly-outlined rectangles indicate the amino acid residues of the synthetic peptide sequence used for synthesizing ovarian LDLR antibody of Mar. japonicus. Reprinted with permission from Zoological Science, 25, Mekuchi et al., Characterization and expression of the putative ovarian lipoprotein receptor in the kuruma prawn, Marsupenaeus japonicus, 428–437, Figs. 1 and 2, © 2008, Zoological Society of Japan.

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