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Fig. 7. Cross-sections of Macrobrachium rosenbergii ovaries. (A) Periodic acid-Schiff (PAS) staining at the endogenous vitellogenic stage. (B) Hematoxylin and eosin (HE) staining at the early exogenous vitellogenic stage. (C) HE staining at the late exogenous vitellogenic stage. (D) PAS staining just after oviposition. (E) PAS staining at the endogenous vitellogenic stage. (F) OsO4 postfixation at the endogenous vitellogenic stage. (G) Immunohistochemistry at the early exogenous vitellogenic stage using anti-vitellin antibody. AO, regressing atretic oocyte; EF, empty follicle after ovulation; EN, endogenous vitellogenic oocyte; EX, exogenous vitellogenic oocyte; O, oogonium; OG, oil globule; PO, previtellogenic oocyte. Bars: 0.1 mm. Reprinted with permission from Fisheries Science, 66, Okumura and Aida, Hemolymph vitellogenin levels and ovarian development during the reproductive and non-reproductive molt cycles in the giant freshwater prawn Macrobrachium rosenbergii, 678–685, Fig. 2, © 2000, The Japanese Society of Fisheries Science.

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