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Fig. 2. Vitellogenin processing in the giant freshwater prawn Macrobrachium rosenbergii (daily changes in subunit composition and schematic diagram). (A) Examination of daily changes in subunit composition of vitellogenin (Vg) in hemolymph and vitellin (Vn) in ovary based on SDS-PAGE and Western blotting in eyestalk ablated female Mac. rosenbergii. Lanes 1–12 (top) correspond to 1–12 days after eyestalk ablation, and show the order of the appearance of Vg bands in the hemolymph. Lanes 1, 4, 8 and 12 (bottom) show corresponding Vn band composition in the ovary during ovarian maturation. (B) Schematic representation of synthesis and processing of vitellogenin in Mac. rosenbergii. Vitellogenin is synthesized as a single precursor molecule, A–B–C/D, in the hepatopancreas, which is then cleaved into two subunits A and proB. Subunits A and proB are released into the hemolymph, where proB is cleaved to form two subunits B and C/D. The three processed subunits A, B and C/D are incorporated into the ovary. Note: as described in the text, VnC and VnD are identical ovarian subunits that showed slight separation on reversed-phase high performance liquid chromaography due to supposed glycosylation. Reprinted with permission of John Wiley & Sons, Inc. from Journal of Experimental Zoology Part A, 292, Okuno et al., The deduced primary structure of vitellogenin in the giant freshwater prawn, Macrobrachium rosenbergii, and yolk processing during ovarian maturation, 417–429, Figs. 4 and 5, © 2002, Wiley-Liss, Inc., a Wiley Company.

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