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Fig. 37. (A) Sagittal section through the brain of barfin flounder on Day 7. Neuronal somata expressing cGnRH-II mRNA are observed in the MT near the ventricle (single arrowheads). (B) Sagittal section through the OE of barfin flounder on Day 21. Neuronal somata expressing sGnRH mRNA are observed in the vicinity of the OE (single arrowhead). (C) Sagittal section through the ON and OB of barfin flounder on Day 28. Neuronal somata expressing sGnRH mRNA are observed in the TN (single arrowheads). (D) Sagittal section through the terminal nerve ganglion of barfin flounder on Day 28. Neuronal somata expressing sGnRH mRNA are observed in the TN (single arrowheads). (E) Sagittal section through the POA of barfin flounder on Day 42. Neurons expressing sbGnRH mRNA are observed (single arrowheads). Bars indicates 20 μm. Reprinted with permission from Zoological Science, 21, Amano et al., Ontogenic development of three GnRH systems in the brain of a pleuronectiform fish, barfin flounder, 311–317, Figs. 3B, 2B, 2D, 2F, 4B, © 2004, Zoological Society of Japan.

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