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Fig. 4. Reverse-phase HPLC of masu salmon brain extract followed by (A) sGnRH RIA and (B) cGnRH-II RIA. Arrows indicate the elution time of synthetic sGnRH and cGnRH-II. The mobile phase was CH3CN (acetonitrile) containing 0.1% TFA. Reprinted with permission from Zoological Science, 9, Amano et al., Changes in salmon GnRH and chicken GnRH-II contents in the brain and pituitary, and GTH contents in the pituitary in female masu salmon, Oncorhynchus masou, from hatching through ovulation. 375–386, Fig. 2, © 1992, Zoological Society of Japan.

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