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Fig. 38. Double immunodiffusion of the extracts of the liver and isolated bacteria against antibody P and puffer liver. 1 and 4: the liver extract of a toxic specimen of the pufferfish Takifugu porphyreus; 2 and 6: the extract of bacteria isolated from the liver, the concentration of the extracts is different; 3 and 5: the extract of antigenic bacteria to prepare antibody P. (a) Center: antibody P, (b): Center the serum of puffer specimen T. porphyreus from which the liver (1 and 4) and bacteria (2 and 6) were prepared. Antibody P was prepared by immunizing rabbit with homogenate of bacteria isolated from the liver of another toxic specimen of T. porphyreus. Reprinted with permission from Kodama, Shimizu, Sato, Ogata and Terao. The infection of bacteria in the liver cells of toxic puffer—A possible cause for organisms to be made toxic by tetrodotoxin in association with bacteria. Harmful Marine Algal BloomsProceed. 6th Intern. Conference Toxic Marine Phytoplankton, Nantes, October 1993, P. Lassus et al., © Technnique et DocumentationLavoisier, 1995.

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