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Fig. 19. HPLC-fluorometric analysis of the extract from Moraxella sp. cells cultured in natural seawater for 10 days at 20°C, using a develosil C8 column according to the method of Oshima et al. (1987). Mobile phase in (A) and (C) is 2 mM 1-heptanesulfonic acid in 10 mM phosphoric acid (pH 7.2). Mobile phase in (B) and (D) is 2 mM 1-heptanesulfonic acid in 10 mM phosphoric acid containing 10% acetonitrile (pH 7.2). (A), GTX standards: GTX1 (5 pmoles); GTX2 (1.3 pmoles); GTX3, (0.5 pmoles); GTX4, (12 pmoles). (B): neoSTX and STX standards; neoSTX, (9 pmoles); STX, (5 pmoles). (C) and (D): extract of Moraxella sp. (10 μL of extract corresponding to cells in 5 mL of culture). Reprinted from Toxicon 28, Kodama et al., Production of paralytic shellfish toxins by a bacterium Moraxella sp. isolated from Protogonyaulax tamarensis. 707–714, © 1990, Pergamon Press plc. with permission from Elsevier.

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