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Fig. 9. Change of toxin components in the mixture of gonyautoxins (GTX) 1,4 and 2-mercaptoethanol (2-ME) during incubation. GTX1,4 (4 mM) was incubated with 1 mL of 8 mM 2-ME in 0.1 M phosphate buffer, pH 7.4, at 70°C. Open square, GTX1,4 in the reaction mixture; closed square, neoSTX in the reaction mixture; open circle, GTX1,4 in the phosphate buffer as a control. Reprinted with permission from Fisheries Science, 66, Sakamoto et al., Formation of intermediate conjugates in the reductive transformation of gonyautoxins to saxitoxins by thiol compounds. 136–141, Fig. 4, © 2000, the Japanese Society of Fisheries Science.

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