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Fig. 2. (A) Eel liver, portal vein, intestine and heart. (B) Polyethylene tubing inserted into portal and hepatic veins. I, Insert polyethylene tubing into the portal vein and fix by ligation. II, Remove a liver with a gall bladder, a part of the intestine and the heart from the body. III, Separate the heart from the liver. Insert another polyethylene tube into the hepatic vein between the heart and the liver, and fix by ligation. Reproduced with permission from Primary culture of eel hepatocytes—Synthesis and secretion of lipoprotein, Hayashi and Komatsu. In: Cell & Tissue Culture: Laboratory Procedures, Doyle, Griffiths and Newell, eds. 1998 © John Wiley & Sons Limited.

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