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Fig. 1. Perfusion apparatus. (I) A, flask (100 mL); B, silicon tubing (100 cm, 2-mm inner diameter, 4-mm outer diameter); C, reservoir; D, three-way cock; E, silicon tubing (15 cm, 4-mm inner diameter, 6-mm outer diameter); F, platform; G, polyethylene tubing for O2 and CO2; a, about 6 cm; b, about 7 cm; 1,2, silicon tubing (1-mm inner diameter, 3-mm outer diameter). (II) A, peristaltic pump; B, reservoir; C, tube for O2 and CO2; D, flask; E, stirrer; F, platform; G, J, thermoregulator; H, hair dryer; I, pH meter; L, liver. Reproduced with permission from Primary culture of eel hepatocytes—Synthesis and secretion of lipoprotein, Hayashi and Komatsu. In: Cell & Tissue Culture: Laboratory Procedures, Doyle, Griffiths and Newell, eds. 1998 © John Wiley & Sons Limited.

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