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Fig. 20. MR cells in gill filaments of killifish acclimated to defined freshwaters with low- (A, D, G), mid- (B, E, H) and high- (C, F, I) NaCl. A­C. Whole-mount preparations stained with anti-Na+/K+-ATPase. D­I. Double immunofluorescence staining of Na+/K+-ATPase (D­F) and V-ATPase (G­I). Bars: A­C, 100 μm; D­I, 50 μm. Reprinted from J. Exp. Biol., 206, Katoh et al., Vacuolar-type proton pump in the basolateral membrane energizes ion uptake in branchial mitochondria-rich cells of killifish Fundulus heteroclitus, adapted to a low ion environment, 793­803, 2003, The Company of Biologists Limited.

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