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Fig. 19. Time-differential double fluorescent staining of branchial MR cells in killifish transferred from seawater (SW) to freshwater (FW) (A, B) and in those maintained in FW (C, D) or in SW (E, F). Gill MR cells were double-labeled in vivo with Rhodamine 123 (A, C, E) just before transfer (day 0) and MitoTracker (B, D, F) on day 3. Cells positive for both Rhodamine 123 and MitoTracker were pre-existing. Arrows indicate newly differentiated MR cells, which are Rhodamine 123-negative (black arrows)/MitoTracker positive (white arrows). Bar: 20 μm. Reprinted from J. Exp. Biol., 206, Katoh and Kaneko, Short-term transformation and long-term replacement of branchial chloride cells in killifish transferred from seawater to freshwater, revealed by morphofunctional observations and a newly established "time-differential double fluorescent staining" technique, 4113­4123, 2003, The Company of Biologists Limited.

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