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Fig. 18. Confocal laser scanning micrographs of whole-mount preparations of the gill filaments (A, C, E, G, I, K) and fluorescence microscope images of gill cryosections (B, D, F, H, J, L) in killifish at 0 h (A, B), 3 h (C, D), 12 h (E, F), 1 day (G, H), 7 days (I, J) and 30 days (K, L) after transfer from seawater to freshwater. Gill filaments were double stained with anti-cystic fibrosis transmembrane conductance regulator (CFTR, green) and anti-Na+/K+-ATPase (red). Bar: 50 μm. Reprinted from J. Exp. Biol., 206, Katoh and Kaneko, Short-term transformation and long-term replacement of branchial chloride cells in killifish transferred from seawater to freshwater, revealed by morphofunctional observations and a newly established "time-differential double fluorescent staining" technique, 4113­4123, 2003, The Company of Biologists Limited.

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