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Fig. 14. Cross sections of killifish embryos and larvae at 10 (A, B), 15 (C, D) and 25 (E, F) days after fertilization, showing the development of the gills. A, C, E. Hematoxylin and eosin staining. B, D, F. Immunocytochemical detection of MR cells with anti-Na+/K+-ATPase. MR cells (arrowheads) are observed in the gill filaments (f) and the opercular membrane (asterisks), but not in the gill lamellae (arrows). a, Gill arch. Bar: 50 μm. Reprinted with permission from Zool. Sci., 17, Katoh et al., Shift of chloride cell distribution during early life stages in seawater-adapted killifish, Fundulus heteroclitus, 11­18, 2000, The Zoological Society of Japan.

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