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Fig. 13. MR cells in the yolk-sac membrane and body skin of killifish embryos and larvae at 2 (A), 4 (B), 8 (C) and 15 days (D, E) after fertilization. MR cells were detected by whole-mount immunocytochemistry with FITC-labeled anti-Na+/K+-ATPase, and observed by conforcal laser scanning microscopy. The ventral view of a larva (E) shows the arrays of MR cells (arrowheads) in the developing gills. Bar: 500 μm. Reprinted with permission from Zool. Sci., 17, Katoh et al., Shift of chloride cell distribution during early life stages in seawater-adapted killifish, Fundulus heteroclitus, 11­18, 2000, The Zoological Society of Japan.

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