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Fig. 12. Double immunofluorescence microscopy of gills in tilapia adapted to freshwater (FW: A, C, E) and seawater (SW: B, D, F), stained with anti-tilapia AQP3 (A, B) and anti-Na+/K+-ATPase as a marker of MR cells (C, D). E and F are merged images of A, C and B, D, respectively. AQP3-immunoreactive cells are observed in both FW and SW gills (A, B), and immunoreactions for AQP3 and Na+/K+-ATPase coincided completely with each other (E, F). Bar: 50 μm. Reprinted from J. Exp. Biol., 208, Watanabe et al., Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater, 2673­2682, 2005, The Company of Biologists Limited.

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