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Fig. 10. A­D. MR cells in the yolk-sac membrane of intact tilapia embryos (A, B) and yolk-ball preparations (C, D) incubated in freshwater (FW: A, C) and seawater (SW: B, D) for 48 h. MR cells were detected by whole-mount immunocytochemistry with FITC-labeled anti-Na+/K+-ATPase. E, F. MR cells in the yolk-sac membrane of yolk-ball preparations incubated in FW (E) and SW (F) for 48 h, double-stained with FITC-labeled anti-Na+/K+-ATPase and propidium iodide. MR cells in SW form multicellular complexes with accessory cells (arrowheads), as demonstrated by the presence of more than one propidium-iodide-labeled nucleus. Bars: 50 μm. Reprinted from J. Exp. Biol., 204, Shiraishi et al., In vitro effects of environmental salinity and cortisol on chloride cell differentiation in embryos of Mozambique tilapia, Oreochromis mossambicus, measured using a newly developed "yolk-ball" incubation system, 1883­1888, 2001, The Company of Biologists Limited.

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