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Fig. 7. A series of differential interference contrast (DIC) and immunofluorescence images of multicellular complexes of MR and accessory cells arranged in the order of presumed developmental stages. A. At first, an undifferentiated cell (uc) is attached to a single, small MR cell (stained green). B, C. The MR cell becomes larger, indented by an accessory cell (ac) originating from the undifferentiated cell. D. The accessory cell is finally enveloped by the MR cell so that the boundary between those cells becomes indistinct. Bar: 10 μm. Reprinted from J. Exp. Biol., 202, Hiroi et al., In vitro sequential changes in chloride cell morphology in the yolk-sac membrane of Mozambique tilapia (Oreochromis mossambicus) embryos and larvae during seawater adaptation, 3485­3495, 1999, The Company of Biologists Limited.

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