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Fig. 5. A. Scanning electron microscopic image of the yolk-sac membrane of a seawater-adapted tilapia larva (blue). B. Distribution image of Ag detected by X-ray microanalysis (red). C. Overlapped image of A and B. X-ray signals, representing the presence of Cl­, are confined to the apical pit (arrow) of the MR cell and its adjacent area. Bars: 5 μm. Reprinted with permission from Kaneko and Shiraishi, Evidence for chloride secretion from chloride cells in the yolk-sac membrane of Mozambique tilapia larvae adapted to seawater, Fish. Sci. 2001, 67, 541­543, Wiley-Blackwell.

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