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Fig. 4. Yolk-sac membranes of seawater (SW)-adapted (A) and freshwater (FW)-adapted (B) tilapia larvae subjected to the chloride test. MR cells (arrowheads) appear as large, round cells when observed with a microscope equipped with a Nomarski's differential interference contrast device. The brown deposition (arrows) is observed in the apical pit of MR cells in SW-adapted larvae, but not in FW-adapted fish. Bar: 50 μm. Reprinted with permission from Kaneko and Shiraishi, Evidence for chloride secretion from chloride cells in the yolk-sac membrane of Mozambique tilapia larvae adapted to seawater, Fish. Sci. 2001, 67, 541­543, Wiley-Blackwell.

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