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Fig. 3. A, C. Transmission electron micrographs of MR cells in the yolk-sac membrane of tilapia larvae (day 3) maintained in freshwater (FW: A) and those transferred from FW to seawater (SW) 1 day before hatching (C). ac, accessory cell; ap, apical pit; pvc, pavement cell; bm, basement membrane. B, D. Magnified views of MR cells in FW (B) and SW (D). Note the numerous mitochondria (m) and well-developed tubular system (t) of MR cells in SW compared with those in FW. E. Horizontal section cut through an apical pit of the multicellular complex of a MR cell in SW. The MR-cell complex consists of a main cell (cc) and an accessory cell (ac), sharing a common apical pit (ap). F. Magnified view of the apical region of the MR-cell complex. The accessory cell interdigitates with the main MR cell and extends cytoplasmic processes (asterisks) toward the apical pit. Note the multiple shallow junctions (arrowheads) between the main and accessory cells. Bars: 1 μm. Reprinted from Cell Tissue Res., 288, 1997, 583­590, Development of multicellular complexes of chloride cells in the yolk-sac membrane of tilapia (Oreochromis mossambicus) embryos and larvae in seawater, Shiraishi et al., Fig. 4. With kind permission of Springer Science+Business Media.

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