TERRAPUB Journal of Oceanography

Journal of Oceanography, Vol. 65 (No. 1), pp. 103-112, 2009

Molecular Ecological Study of Siganus spinus and S. guttatus from Okinawan Waters Based on Mitochondrial DNA Control Region Sequences

Kensuke Iwamoto1, Akihiro Takemura2, Tetsuo Yoshino3 and Hideyuki Imai3*

1Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan
2Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa 905-0227, Japan
3Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan

(Received 28 December 2007; in revised form 21 June 2008; accepted 22 June 2008)

Abstract: The mitochondrial DNA (mtDNA) control region was sequenced to examine the genetic variability and gene flow of juvenile Siganus spinus and S. guttatus. In total, 461 nucleotide sequences were obtained from 69 specimens of juvenile S. spinus from Okinawa Island and Ishigaki Island, in which 17 variable sites and 22 haplotypes were identified. Haplotype diversity (h) was high (0.9244 in Okinawa and 0.8984 in Ishigaki), whereas nucleotide diversity (π) was low (0.0063 in Okinawa and 0.0059 in Ishigaki). The two populations were not genetically distinct. Siganus guttatus, which do not form large schools at recruitment, in contrast S. spinus, were also analyzed by studying 152 individuals collected off Okinawa Island, Miyako Island, and Ishigaki Island. Of 476 nucleotide sequences, 50 were variable, and 42 haplotypes were identified. Genetic variability values were high (h = 0.8766 and π = 0.0151 in Okinawa; h = 0.9640 and π = 0.0192 in Miyako; h = 0.9161 and π = 0.0199 in Ishigaki). The Okinawa population was genetically isolated from the Miyako and Ishigaki populations. As a result, genetic diversity was high for each of these siganid populations despite their being target species for fisheries; however, the degree of inter-population gene flow was higher for S. spinus than S. guttatus, suggesting that these species exhibit different dispersal strategies.

*Corresponding author E-mail: imai@sci.u-ryukyu.ac.jp

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