TERRAPUB Journal of Oceanography

Journal of Oceanography, Vol. 57 (No. 1), pp. 109-113, 2001

Short Contribution

Gram Stain Index (GSI) of Bacterial and Archaeal Cells in the Natural Microbial Communities of Slightly and Extremely Saline Environments

Haruo Saida1*, Masahiro Kamekura2, Wael S. M. El-Sayed3, Mohamed Abu-Shady3, Yukuo Abe1, Tomoharu Yamaguchi1, Peiling Yang4, Takaaki Maekawa1 and Humitake Seki5

1Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
2Noda Institute for Scientific Research, 399 Noda, Chiba 278-0037, Japan
3Microbiology Deparment, Faculty of Science, Ain Shams University, Cairo, Egypt
4Depatment of Water Conservancy Engineering, China Agriculture University, Qinghua, Donglu, Beijing 100083, P.R. China
5Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

(Received 30 April 2000; in revised form 9 July 2000; accepted 31 August 2000)

Abstract: The use of GSI (Gram Stain Index) was studied for natural microbial cells in slightly and extremely saline environments. The GSI spectra of archaeal cells in an extremely saline environment showed unique profiles, different from those of Bacteria. These profiles can be applied for structural analysis of the microbial communities in aquatic environments.

*Corresponding author E-mail: saida@bsys.tsukuba.ac.jp

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